Electron microscopy of peripheral nerve fibers.

نویسنده

  • R HONJIN
چکیده

Considerable information on the submicroscopic structure of nerve fibers has been obtained from polarization opitical analyses and x-ray diffraction studies, but such methods do not allow direct visualization of details lying beyond the light microscope's resolution . Successful application of the electron microscope to this problem with its increase in resolving power has made possible an extension of our knowlege of the ultrastructure of nerve fibers. As in the cases of other tissues , the electron microscope studies of the nerve fibers have been examined by means of two methods, i. e., technique of maceration and that of cutting ultrathin sections. Some of the previous researchers , i. e., Hess and Lansing (1953), have considered that the technique of maceration would be perhaps best not applied to mammalian nerve fibers, because the isolated elements are difficult to trace back to their original position within the nerve fiber. It seems, however, that the fragmentation procedures of nerve fibers afford an incomparable advantage for detailed discussion about them, though the greatest care must be exercised against misconstruction. Accordingly it is advisable to study the same materials with these two different methods. In the present paper , we intend to account for the submicroscopic structure of peripheral nerve fibers, utilizing the electron microscope with the macerating materials as well as with the thin sections.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of calcium phosphate cement on the peripheral nerve fibers.

Calcium phosphate cement (CPC) is a bioactive ceramic substance. To clarify the effects of CPC on the peripheral nerve, we applied CPC on the peripheral nerve fibers of experimental animals and investigated the nerve fibers by electron microscopy and by immunoblotting analysis using an anti-myelin-associated glycoprotein (anti-MAG) antibody. The results showed that there was neither axonal spro...

متن کامل

Schwann cell glycogen selectively supports myelinated axon function.

OBJECTIVE Interruption of energy supply to peripheral axons is a cause of axon loss. We determined whether glycogen was present in mammalian peripheral nerve, and whether it supported axon conduction during aglycemia. METHODS We used biochemical assay and electron microscopy to determine the presence of glycogen, and electrophysiology to monitor axon function. RESULTS Glycogen was present i...

متن کامل

Morphological Identification of Cell Death in Dorsal Root Ganglion Neurons Following Peripheral Nerve injury and repair in adult rat

Background: Axotomy causes sensory neuronal loss. Reconnection of proximal and distal nerve ends by surgical repair improves neuronal survival. It is important to know the morphology of primary sensory neurons after the surgical repair of their peripheral processes. Methods: Animals (male Wistar rats) were exposed to models of sciatic nerve transection, direct epineurial suture repair of sciati...

متن کامل

Light and electron microscopic study of peripheral nerve damage in patients with lepromatous leprosy (LL) and borderline lepromatous leprosy (BL).

Cutaneous branches of radial nerves in patients with lepromatous leprosy (LL) and borderline lepromatous (BL) were studied by light and electron microscopy. Foamy macrophages were found more or less in the nerve fibers of all leprosy patients and distributed in the epineurial, perineurial and endoneurial areas. In the endoneurium, the foamy macrophages were mainly located in the subperineurial ...

متن کامل

Stress and strain analysis on the anastomosis site sutured with either epineurial or perineurial sutures after simulation of sciatic nerve injury☆

The magnitude of tensile stress and tensile strain at an anastomosis site under physiological stress is an important factor for the success of anastomosis following suturing in peripheral nerve injury treatment. Sciatic nerves from fresh adult cadavers were used to create models of sciatic nerve injury. The denervated specimens underwent epineurial and perineurial suturing. The elastic modulus ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Okajimas folia anatomica Japonica

دوره 27 4  شماره 

صفحات  -

تاریخ انتشار 1955